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Protective Effect of Hydro-Alcoholic Extract of Ruta Graveolens Linn. Leaves on Indomethacin and Pylorus Ligation-Induced Gastric Ulcer in Rats

Protective Effect of Hydro-Alcoholic Extract of Ruta Graveolens Linn. Leaves on Indomethacin and Pylorus Ligation-Induced Gastric Ulcer in Rats

Mohd Tarique, Hefazat H Siddiqui, Mohd Khushtar, Md Azizur Rahman

J Ayurveda Integr Med. 2016 Mar;7(1):38-43.

PMID: 27297508

Abstract:

Background:
The search for an ideal and new antiulcer drug has been extended to herbals for novel molecules that decrease the incidence of relapse and afford better protection.
Objective:
The present study was designed to investigate the protective effect of hydro-alcoholic extract of Ruta graveolens (RGE) Linn. leaves on indomethacin (IND) and pylorus ligation-induced gastric ulcer in Wistar rats.
Materials and methods:
The rats of all the six groups were deprived of food for 24 h. Then, the first group received 1 ml/kg/day p.o. of 1% carboxymethylcellulose calcium (CMC), second group 1 ml/kg/day p.o. of 1% CMC and third group 20 mg/kg/day p.o. of IND. Fourth and fifth groups received RGE 200 and 400 mg/kg/day p.o., respectively; while the sixth group 10 mg/kg/day p.o. omeprazole. After 30 min, last three groups received 20 mg/kg/day p.o. of IND also. All these treatments after food deprivation were repeated each day for 5 consecutive days. Pylorus ligation was performed on 6th day in last five groups. After 4 h, stomach by sacrifice of the rats was examined for ulcer index (UI) and gastric mucus. Gastric juice was assessed for acidity, pH and pepsin; while gastric tissues were assessed for thiobarbituric acid reactive substance (TBARS) and glutathione (GSH).
Results:
Fifth group showed significant decrease in UI (10.33 ± 0.67), TBARS (0.33 ± 0.03 mmol/mg), free acidity (48.78 ± 5.12 meq/l/100 g), total acidity (99.33 ± 9.31 meq/l/100 g), and pepsin activity (8.47 ± 0.41 μg/ml) levels while it showed significant increase in mucus (412.4 ± 21.6 μg/g), GSH (57.9 ± 4.8 mmol/mg) and pH (3.32 ± 0.27) compared to third group. Percent protection in RGE 400 mg was found to be 63.32 compared to indomethacin.
Conclusion:
RGE possesses antiulcerogenic activity as it exhibits protective effect on gastric ulcer in rats.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9050048	Carboxymethylcellulose calcium		9050-04-8	Price

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