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Protective Effects of Poly(lactic-Co-Glycolic Acid) Nanoparticles Loaded With Erythropoietin Stabilized by Sodium Cholate Against Glutamate-Induced Neurotoxicity

Protective Effects of Poly(lactic-Co-Glycolic Acid) Nanoparticles Loaded With Erythropoietin Stabilized by Sodium Cholate Against Glutamate-Induced Neurotoxicity

Ji Heun Jeong, Seung Hee Kang, Jeong Hwan Kim, Kwang Sik Yu, In Ho Lee, Ye Ji Lee, Je Hun Lee, Nam Seob Lee, Young Gil Jeong, Do Kyung Kim, Gyu Hyun Kim, Shin Hye Lee, Seul Ki Hong, Seung-Yun Han, Bo Sun Kang

J Nanosci Nanotechnol. 2014 Nov;14(11):8365-71.

PMID: 25958529

Abstract:

The final aim of this study was to confirm the neuroprotective effects of recombinant human erythropoietin (rhEPO)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles stabilized by sodium cholate (rhEPO-Ch-NP) and compare their effects with those of rhEPO using an in vitro model of cerebral ischemia. Glutamate-induced excitotoxic damage on SH-SY5Y cells, a human neuroblastoma cell line, with or without rhEPO-Ch-NPs was quantitatively evaluated. The rhEPO-Ch-NPs were carefully prepared using a water-in-oil-in-water (w/o/w) emulsion solvent evaporation technique with PLGA, sodium cholate hydrate, and ethyl acetate. The rhEPO-Ch-NPs were fully characterized by both transmission electron microscopy (TEM) and differential scanning calorimetry (DSC). In addition, significant intracellular uptake of these particles was monitored by confocal microscopy. Notably, the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and nuclear changes observed by 4',6-diamidino-2-phenylindole (DAPI) staining in SH-SY5Y cells demonstrated that rhEPO-Ch-NPs were safer at any concentration investigated and rescued more neuronal cells, while preserving normocytic features against glutamate-induced excitotoxic damages compared to rhEPO.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP206986870	Sodium cholate hydrate		206986-87-0	Price

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