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Protein A-peroxidase: A Valluable Tool for the Localization of Antigens

Protein A-peroxidase: A Valluable Tool for the Localization of Antigens

M Dubois-Dalcq, H McFarland, D McFarlin

J Histochem Cytochem. 1977 Nov;25(11):1201-6.

PMID: 199666

Abstract:

Protein A of Staphylococcus aureus has been conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. The same Protein A-peroxidase conjugate was used with antisera from five different species. Using this indirect test, membrane markers for T and B lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. Viral antigens on cells infected with measles, vesicular stomatitis, herpes or visna virus, respectively, were also stained in the protein A-peroxidase indirect test with a greater specificity than indirect method using anti-immunoglobulin. Paired preparations were examined in the light and electron microscope. Ultrastructural analysis showed that the protein A-peroxidase conjugate penetrated well through fixed viral membranes and resulted in fine resolution of antigenic sites.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411211	Protein A-Peroxidase from Staphylococcus aureus/horseradish			Price

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