Home
Resources
Publications
Proteinase-activated Receptor 2 (PAR(2)) in Cholangiocarcinoma (CCA) Cells: Effects on Signaling and Cellular Level

Proteinase-activated Receptor 2 (PAR(2)) in Cholangiocarcinoma (CCA) Cells: Effects on Signaling and Cellular Level

Roland Kaufmann, Alexander Hascher, Franziska Mussbach, Petra Henklein, Kathrin Katenkamp, Martin Westermann, Utz Settmacher

Histochem Cell Biol. 2012 Dec;138(6):913-24.

PMID: 22892662

Abstract:

In this study, we demonstrate functional expression of the proteinase-activated receptor 2 (PAR(2)), a member of a G-protein receptor subfamily in primary cholangiocarcinoma (PCCA) cell cultures. Treatment of PCCA cells with the serine proteinase trypsin and the PAR(2)-selective activating peptide, furoyl-LIGRLO-NH(2), increased migration across a collagen membrane barrier. This effect was inhibited by a PAR(2)-selective pepducin antagonist peptide (P2pal-18S) and it was also blocked with the Met receptor tyrosine kinase (Met) inhibitors SU 11274 and PHA 665752, the MAPKinase inhibitors PD 98059 and SL 327, and the Stat3 inhibitor Stattic. The involvement of Met, p42/p44 MAPKinases and Stat3 in PAR(2)-mediated PCCA cell signaling was further supported by the findings that trypsin and the PAR(2)-selective agonist peptide, 2-furoyl-LIGRLO-NH(2), stimulated activating phosphorylation of these signaling molecules in cholangiocarcinoma cells. With our results, we provide a novel signal transduction module in cholangiocarcinoma cell migration involving PAR(2)-driven activation of Met, p42/p44 MAPKinases and Stat3.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP658084232-B	SU 11274		658084-23-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: