Home
Resources
Publications
Proteolytic Activity of Proteinases on Macropeptide Isolated From Kappa-Casein

Proteolytic Activity of Proteinases on Macropeptide Isolated From Kappa-Casein

K M Shammet, R J Brown, D J McMahon

J Dairy Sci. 1992 Jun;75(6):1380-8.

PMID: 1500545

Abstract:

Proteolytic activities of chymosin, bovine pepsin, Mucor miehei rennet, Cryphonectria parasitica (formerly Endothia parasitica) rennet, trypsin, and chymotrypsin on kappa-casein macropeptide were measured. Macropeptide solutions (10 mg/ml of .05 M, pH 6.6 phosphate buffer) were incubated with the enzymes at 37 degrees C for various times, and their reactions were stopped by adding .025 ml of pepstatin (1 mg/ml of methanol). Peptides released from kappa-casein macropeptide were then fractionated using reverse-phase HPLC. At the pH of milk (pH 6.6), kappa-casein macropeptide was resistant to enzymic action by chymosin, bovine pepsin, and M. miehei and C. parasitica rennets. Bovine pepsin hydrolyzed kappa-casein macropeptide at pH 3. kappa-Casein macropeptide was readily hydrolyzed at pH 6.6 by trypsin and chymotrypsin. Possible physiological functions of the kappa-casein macropeptide are discussed in light of these findings.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9042084	Rennet from Mucor miehei		9042-08-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: