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Protocol for Apoptosis Assay by Flow Cytometry Using Annexin V Staining Method

Protocol for Apoptosis Assay by Flow Cytometry Using Annexin V Staining Method

Imayavaramban Lakshmanan, Surinder K Batra

Bio Protoc. 2013 Mar 20;3(6):e374.

PMID: 27430005

Abstract:

This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium Iodide solution followed by flow cytometry analysis. It is based on the principle that normal cells are hydrophobic in nature as they express phosphatidyl serine in the inner membrane (side facing the cytoplasm) and when the cells undergo apoptosis, the inner membrane flips to become the outer membrane, thus exposing phosphatidyl serine. The exposed phosphatidyl serine is detected by Annexin V, and propidium iodide stains the necrotic cells, which have leaky DNA content that help to differentiate the apoptotic and necrotic cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25535164-C	Propidium iodide solution		25535-16-4	Price

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