Home
Resources
Publications
Proton NMR Studies of Apo-Neocarzinostatin From Streptomyces Carzinostaticus. Sequence-specific Assignment and Secondary Structure

Proton NMR Studies of Apo-Neocarzinostatin From Streptomyces Carzinostaticus. Sequence-specific Assignment and Secondary Structure

E Adjadj, J Mispelter, E Quiniou, J L Dimicoli, V Favaudon, J M Lhoste

Eur J Biochem. 1990 Jun 20;190(2):263-71.

PMID: 2142075

Abstract:

The sequence-specific resonance assignment of apo-neocarzinostatin from Streptomyces carzinostaticus was carried out from two-dimensional proton-NMR spectra. The assignments were obtained for the backbone protons of 111 of the 113 residues of the protein, missing the two C alpha H of one glycine but including 3 of the 4 prolines. The majority of side chain protons were also assigned. The secondary structure derived from the analysis of sequential connections corresponds to ten beta-strands separated by clearly identified loops and turns. Inter-strand connectivities and slowly exchanging amide protons confirm the presence of the two disulfide bridges from Cys37 to Cys47 and from Cys88 to Cys93 and indicate a global folding similar to that of the similar proteins, actinoxanthin and macromomycin, for which crystallographic data are available.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9014022	Neocarzinostatin from Streptomyces carzinostaticus		9014-02-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: