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Purification and Characterization of a β-glucosidase From Aspergillus Niger and Its Application in the Hydrolysis of Geniposide to Genipin

Purification and Characterization of a β-glucosidase From Aspergillus Niger and Its Application in the Hydrolysis of Geniposide to Genipin

Guohong Gong, Zhiming Zheng, Hui Liu, Li Wang, Jinshan Diao, Peng Wang, Genhai Zhao

J Microbiol Biotechnol. 2014 Jun 28;24(6):788-94.

PMID: 24608563

Abstract:

An extracellular β-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 β-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at 65°C and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The β-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 β-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9033061	Glucosidase from Aspergillus niger		9033-06-1	Price

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