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Purification and Characterization of Cystatin From Duck Egg White

Purification and Characterization of Cystatin From Duck Egg White

M Warwas, J Gburek, J Osada, K Gołab

Acta Biochim Pol. 1995;42(3):351-6.

PMID: 8588488

Abstract:

It is the second peptidase inhibitor, after ovostatin, which showing the same antipapain activity in egg white in different avian species implies differences in amino-acid sequences. Cystatin from duck egg white was purified by carboxymethylpapain affinity chromatography and size-exclusion HPLC. The purified inhibitor which showed partial identity in the immunodiffusion test with chicken egg white cystatin, had an apparent molecular mass of 9.3 kDa as determined by SDS/PAGE. IEF analysis revealed five molecular forms of pI in the range 7.8-8.4. The obtained cystatin was neither glycosylated nor phosphorylated as it is in the case of chicken cystatin. The determined Ki (0.005 +/- 0.001 nM) was similar to that reported for human and chicken cystatin C.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP81989959	Cystatin from chicken egg white		81989-95-9	Price

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