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Purification and Characterization of Purine Nucleoside Phosphorylase and Pyrimidine Nucleoside Phosphorylase From Bacillus Stearothermophilus TH 6-2

T Hamamoto, T Noguchi, Y Midorikawa

Biosci Biotechnol Biochem. 1996 Jul;60(7):1179-80.

PMID: 8782414

Abstract:

The purine nucleoside phosphorylase (Pu-NPase) and the pyrimidine nucleoside phosphorylase (Py-NPase) have been purified from Bacillus stearothermophilus TH 6-2. The Pu-NPase is a trimer of 30-kDa subunits and the Py-NPase is a dimer of 46-kDa subunits. The isoelectric points of Pu-NPase and Py-NPase were pH 4.3 and 4.6, respectively. The Pu-NPase could catalyze the phosphorolysis of inosine and guanosine, but not adenosine. the Py-NPase could phosphorolyze both uridine and thymidine.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9055350 Pyrimidine nucleoside phosphorylase Pyrimidine nucleoside phosphorylase 9055-35-0 Price
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