Home
Resources
Publications
Purification of Antibodies: Diethylaminoethyl (DEAE) Chromatography

Purification of Antibodies: Diethylaminoethyl (DEAE) Chromatography

Jordan B Fishman, Eric A Berg

Cold Spring Harb Protoc. 2019 Jan 2;2019(1).

PMID: 30602557

Abstract:

Because IgG from most species (other than rodents) tends to have an isoelectric point around neutral, two approaches can be used when separating IgG using diethylaminoethyl (DEAE) resins. When serum containing antibodies is applied to DEAE at a slightly acidic pH, the IgG flows through the column while most other serum proteins bind to the DEAE. This method is best performed using a batch method. The DEAE beads can be kept in a disposable syringe containing a polypropylene frit, a glass reactor containing a coarse-sintered glass frit, or other suitable vessel. If the antibody solution is adjusted to a basic pH of 8-8.5, then IgG binds to the DEAE resin. After washing the column, the antibody is eluted by adding a buffer of increasing ionic strength to the column. Prepacked columns of many sizes are available for the isolation of antibodies by DEAE chromatography. Alternatively, DEAE medium can be swelled or prepped according to the manufacturer's instructions and a column can be poured when needed.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
TIT9302	CombiCoulomat frit			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: