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Purification of Bovine Lens and Bacterially Expressed Human Vimentin

Edward R Kuczmarski, Robert D Goldman

CSH Protoc. 2007 Feb 1;2007:pdb.prot4675.

PMID: 21357021

Abstract:

INTRODUCTIONIntermediate filaments (IF) are major cytoskeletal systems of vertebrate and many nonvertebrate cells whose expression is cell-type specific and developmentally regulated. This protocol describes a method for purifying one type of IF, vimentin, from bovine lens tissue. Purification of human vimentin expressed in Escherichia coli is also described. These methods are useful in the preparation of other IF protein subunits for microinjection studies as well.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP88566409 Vimentin from bovine lens Vimentin from bovine lens 88566-40-9 Price
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