0

Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments

Giampiero Pietrocola, Simonetta Rindi, Giulia Nobile, Pietro Speziale

Methods Mol Biol. 2017;1627:309-324.

PMID: 28836210

Abstract:

A method is described for the purification of plasma fibronectins based on a combination of gelatin- and arginine-Sepharose chromatography steps. Cellular fibronectin can be purified from an osteosarcoma fibroblast cell line by affinity chromatography using a monoclonal antibody anti-fibronectin as ligand. Furthermore, we also provide a protocol for the purification of fibronectin domains obtained by fractionation of thermolysin-digested plasma fibronectin on ion-exchange/gel filtration chromatography columns. Assessment of the fibronectin purity is performed by SDS-PAGE, while the ligand binding activities of specific fibronectin domains are determined by ELISA.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR4241156 Fibronectin human plasma Fibronectin human plasma Price
qrcode