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Purification of Insoluble Nitric Oxide Synthase From Rat Cerebellum

Purification of Insoluble Nitric Oxide Synthase From Rat Cerebellum

K Hiki, R Hattori, C Kawai, Y Yui

J Biochem. 1992 May;111(5):556-8.

PMID: 1379222

Abstract:

Nitric oxide synthase [EC 1.14.23] from the particulate fraction of rat cerebella was purified and characterized. The homogenate of rat cerebella was centrifuged to obtain a pellet, which was washed and incubated with Triton X-100 containing buffer. The enzyme activity appeared in the 100,000 x g supernatant after incubation with the detergent. The solubilized enzyme was then purified by sequential affinity chromatography using adenosine 2',5'-diphosphate agarose and calmodulin Sepharose 4B, which gave a product that migrated as a single protein band on SDS/PAGE with a molecular mass of about 150 kDa. The purified enzyme exhibited an absolute requirement for FAD, in addition to NADPH and Ca2+/calmodulin. Thus, there is an insoluble nitric oxide synthase in rat cerebellum that has similar characteristics to the soluble type.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411037	Adenosine 2′,5′-diphosphate-Agarose			Price

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