0

Purification of Lysozyme From Human Neutrophils, and Development of an ELISA for Quantification in Cells and Plasma

K Lollike, L Kjeldsen, H Sengeløv, N Borregaard

Leukemia. 1995 Jan;9(1):206-9.

PMID: 7845019

Abstract:

Lysozyme was purified from exocytosed granule material from PMA-stimulated human neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and plasma.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9001632-A Lysozyme from human neutrophils Lysozyme from human neutrophils 9001-63-2 Price
qrcode