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Purification of the Crude Solution From Helix Pomatia for Use as Beta-Glucuronidase and Aryl Sulfatase in Phytoestrogen Assays

Purification of the Crude Solution From Helix Pomatia for Use as Beta-Glucuronidase and Aryl Sulfatase in Phytoestrogen Assays

Philip B Grace, Philip Teale

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 17;832(1):158-61.

PMID: 16388994

Abstract:

Phytoestrogens occur in a variety of foods and are thought to offer a protective effect against a number of complex diseases. Due to the diversity of phytoestrogen conjugates formed in the human body, most assays include an enzymatic hydrolysis step prior to analysis. beta-Glucuronidase from Helix pomatia, which also contains sulfatase activity, is popular for this task but contains appreciable levels of some phytoestrogens and related compounds, which could affect accurate quantification at low concentrations. Use of solid phase extraction on a polymeric resin has been found to remove the majority of these compounds from the enzyme, without affecting the enzyme activity for almost all of the analytes tested.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001450-B	β-Glucuronidase from Helix pomatia		9001-45-0	Price

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