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Purification, Quantification, and Functional Analysis of Collectins

Katsuki Ohtani, Nobutaka Wakamiya

Methods Mol Biol. 2020;2132:99-109.

PMID: 32306318

Abstract:

Native and recombinant collectins are purified by using mannan-agarose and an anti-collectin antibody column. The use of sandwich enzyme-linked immunosorbent assay (ELISA) with two antibodies against human mannan-binding lectin (MBL) enables elucidation of the collectin concentration in the blood, serum, and plasma. The collectin sugar specificity is demonstrated by determining the concentration of saccharide required to inhibit sugar binding by 50% in a saccharide-binding assay. Biological analyses including the complement-dependent hemolysis test and several other methods are used to evaluate collectin.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42410968 Mannan−Agarose Mannan−Agarose Price
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