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Quality Assessment of Traditional Herbal Formula, Hyeonggaeyeongyo-tang Through Simultaneous Determination of Twenty Marker Components by HPLC-PDA and LC-MS/MS

Quality Assessment of Traditional Herbal Formula, Hyeonggaeyeongyo-tang Through Simultaneous Determination of Twenty Marker Components by HPLC-PDA and LC-MS/MS

Chang-Seob Seo, Hyeun-Kyoo Shin

Saudi Pharm J. 2020 Apr;28(4):427-439.

PMID: 32273801

Abstract:

Simultaneous analysis of 20 marker components (gallic acid, cimifugin, geniposide, paeoniflorin, ferulic acid, nodakenin, narirutin, naringin, neohesperidin, arctiin, baicalin, oxypeucedanin hydrate, wogonoside, baicalein, arctigenin, glycyrrhizin, wogonin, pulegone, decursin, and decursinol angelate) for quality assessment of the traditional herbal formula, Hyeonggaeyeongyo-tang (HYT) was carried out by using high-performance liquid chromatography (HPLC) with photodiode array detection (PDA) and liquid chromatography-mass spectrometry with tandem mass spectrometry (LC-MS/MS). The coefficient of determination showed excellent linearity of more than 0.9999 for all analytes. The recovery of 20 marker components was 93.92 to 102.66% with relative standard deviation (RSD) < 3.00% and RSD value of precision was ≤ 3.44%. The amounts of 20 marker components using HPLC-PDA and LC-MS/MS were determined to be 0.18-14.60 and 0.01-1.76 mg/freeze-dried g, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
ALP632859	Wogonin hydrate		632-85-9 (anhydrous)	Price

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