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Quantification of Ascorbyl Adducts of Epigallocatechin Gallate and Gallocatechin Gallate in Bottled Tea Beverages

Quantification of Ascorbyl Adducts of Epigallocatechin Gallate and Gallocatechin Gallate in Bottled Tea Beverages

Wei-Lun Hung, Siyu Wang, Shengmin Sang, Xiaochun Wan, Yu Wang, Chi-Tang Ho

Food Chem. 2018 Sep 30;261:246-252.

PMID: 29739590

Abstract:

Catechins are the major bioactive compounds existing in tea leaves (Camellia sinensis). Dehydroascorbic acid is (DHAA) a reactive dicarbonyl species and previous studies have demonstrated that catechins could effectively trap DHAA to form ascorbyl adducts of catechins, especially epigallocatechin gallate (EGCG). Since catechins in the aqueous solution are unstable due to their structural features, ascorbic acid (AA) is usually added to bottled tea beverages to protect catechins. However, whether ascorbyl adducts of catechins are formed in bottled tea beverages remains unclear. In this study, formation of ascorbyl adducts of EGCG increased along with increased incubation time when EGCG and AA were dissolved in the aqueous solution. Next, 6C-DHAA-EGCG and 8C-DHAA-EGCG were detected in both green tea and oolong tea beverages, and their concentrations ranged from 0.23 to 1.95 µM and 0.28 to 1.97 µM, respectively. Furthermore, an 8C-ascorbyl adduct derived from gallocatechin gallate was also found in some tea beverages.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4233969	(−)-Gallocatechin gallate		4233-96-9	Price

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