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Quantification of Bupivacaine Hydrochloride and Isoflupredone Acetate Residues in Porcine Muscle, Beef, Milk, Egg, Shrimp, Flatfish, and Eel Using a Simplified Extraction Method Coupled With Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

Quantification of Bupivacaine Hydrochloride and Isoflupredone Acetate Residues in Porcine Muscle, Beef, Milk, Egg, Shrimp, Flatfish, and Eel Using a Simplified Extraction Method Coupled With Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

Sang-Hyun Cho, Jin-A Park, Weijia Zheng, A M Abd El-Aty, Seong-Kwan Kim, Jeong-Min Choi, Hee Yi, Soo-Min Cho, Nehal A Afifi, Jae-Han Shim, Byung-Joon Chang, Jin-Suk Kim, Ho-Chul Shin

J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Oct 15;1065-1066:29-34.

PMID: 28946122

Abstract:

In this study, a simple analytical approach has been developed and validated for the determination of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A 0.1% solution of acetic acid in acetonitrile combined with n-hexane was used for deproteinization and defatting of all tested matrices and the target drugs were well separated on a Waters Xbridge™ C18 analytical column using a mobile phase consisting of 0.1% acetic acid (A) and 0.1% solution of acetic acid in methanol (B). The linearity estimated from six-point matrix-matched calibrations was good, with coefficients of determination ≥0.9873. The limits of quantification (LOQs) for bupivacaine hydrochloride and isoflupredone acetate were 1 and 2ngg-1, respectively. Recovery percentages in the ranges of 72.51-112.39% (bupivacaine hydrochloride) and 72.58-114.56% (isoflupredone acetate) were obtained from three different fortification concentrations with relative standard deviations (RSDs) of <15.14%. All samples for the experimental work and method application were collected from the local markets in Seoul, Republic of Korea, and none of them tested positive for the target drugs. In conclusion, a simple method using a 0.1% solution of acetic acid in acetonitrile and n-hexane followed by LC-MS/MS could effectively extract bupivacaine hydrochloride and isoflupredone acetate from porcine muscle, beef, milk, egg, shrimp, flatfish, and eel samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP338987	Isoflupredone acetate		338-98-7	Price

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