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Quantification of Neomangiferin in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to Bioavailability Study

Quantification of Neomangiferin in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to Bioavailability Study

Bo Yang, Zhirui Liu, Shenglan Shang, Xiaojian Qin, Peiyuan Xia

J Pharm Anal. 2016 Oct;6(5):335-340.

PMID: 29404001

Abstract:

Neomangiferin, a natural C-glucosyl xanthone, has recently received a great deal of attention due to its multiple biological activities. In this study, a rapid and sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for the quantification of neomangiferin in rat plasma was developed. Using chloramphenicol as an internal standard (IS), plasma samples were subjected to a direct protein precipitation process using methanol (containing 0.05% formic acid). Quantification was performed by multiple reactions monitoring (MRM) method, with the transitions of the parent ions to the product ions of m/z 583.1→330.9 for NG and m/z 321.1→151.9 for IS. The assay was shown to be linear over the range of 0.2-400 ng/mL, with a lower limit of quantification of 0.2 ng/mL. Mean recovery of neomangiferin in plasma was in the range of 97.76%-101.94%. Relative standard deviations (RSDs) of intra-day and inter-day precision were both <10%. The accuracy of the method ranged from 94.20% to 108.72%. This method was successfully applied to pharmacokinetic study of neomangiferin after intravenous (2 mg/kg) and intragastric (10 mg/kg) administration for the first time. The oral absolute bioavailability of neomangiferin was estimated to be 0.53%±0.08% with an elimination half-life (t1/2) value of 2.74±0.92 h, indicating its poor absorption and/or strong metabolism in vivo.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP64809672	Neomangiferin		64809-67-2	Price

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