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Quantification of Polycyclic Aromatic Hydrocarbons (PAHs) in Human Hair by HPLC With Fluorescence Detection: A Biological Monitoring Method to Evaluate the Exposure to PAHs

Quantification of Polycyclic Aromatic Hydrocarbons (PAHs) in Human Hair by HPLC With Fluorescence Detection: A Biological Monitoring Method to Evaluate the Exposure to PAHs

Akira Toriba, Yayoi Kuramae, Thaneeya Chetiyanukornkul, Ryoichi Kizu, Tsunehisa Makino, Hiroyuki Nakazawa, Kazuichi Hayakawa

Biomed Chromatogr. Mar-Apr 2003;17(2-3):126-32.

PMID: 12717801

Abstract:

A high-performance liquid chromatographic (HPLC) method with fluorescence detection was developed for the quantification of polycyclic aromatic hydrocarbons (PAHs) in human hair. Fifteen kinds of PAHs classified as priority pollutants by the US EPA were quantified with four perdeuterated PAHs as internal standards. After 50 mg hair samples were washed with n-hexane to remove external contamination of PAHs, the samples were digested in 2.5 M sodium hydroxide. The digests were extracted with n-hexane and then analyzed by HPLC. Eleven kinds of PAHs were identified in hair samples of 20 subjects, and 10 kinds of PAHs were eventually quantified using the internal standards. For anthracene, chrysene and benzo[k]fluoranthene, significant differences were observed between smokers and non-smokers. Although benzo[b]fluoranthene, dibenz[a,h]anthracene, benzo[ghi]perylene and indeno[1,2,3-cd]pyrene were observed in the particulates of indoor and outdoor air, they were not detected in all hair samples. The analysis of PAHs in human hair should be useful as a new biomarker to evaluate the exposure to PAHs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS26686	PAHs by HPLC			Price

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