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Quantification of Seven nucleoside/nucleotide Reverse Transcriptase Inhibitors in Human Plasma by High-Performance Liquid Chromatography With Tandem Mass-Spectrometry

Quantification of Seven nucleoside/nucleotide Reverse Transcriptase Inhibitors in Human Plasma by High-Performance Liquid Chromatography With Tandem Mass-Spectrometry

Thomas Le Saux, Stéphanie Chhun, Elisabeth Rey, Odile Launay, Laurence Weiss, Jean-Paul Viard, Gérard Pons, Vincent Jullien

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):81-90.

PMID: 18325857

Abstract:

A simple analytical method was developed in 100 microL of plasma for the simultaneous assay of the 7 nucleoside/nucleotide reverse transcriptase inhibitors (abacavir, didanosine, emtricitabine, lamivudine, stavudine, tenofovir, and zidovudine) currently used for the treatment of HIV-infected patients. After adding the internal standard, 6-beta-hydroxy-theophyline, plasma samples were precipitated with 500 microL acetonitrile and the supernatants were evaporated to dryness. The residues were reconstituted with 500 microL of water and 10 microL of the extracts were injected in the chromatographic system. The chromatographic separation was performed with a C-18 column and a gradient mobile phase consisting of a mixture of water and acetonitrile, both containing 0.05% formic acid. Analytes quantification was performed by electrospray ionisation triple quadrupole mass-spectrometry in the positive mode using selected reaction monitoring (SRM). Intra- and inter-assay precision and accuracy were lower than 20% for the limit of quantification, and 15% for higher concentrations. The method has been implemented to assess plasma concentrations of patients infected by HIV and was found suitable for therapeutic drug monitoring.

Chemicals Related in the Paper:

Catalog Number	Product Name	Price
CS31041357	Didanosine System Suitability Mixture	Price
CS31041408	Emtricitabine System Suitability Mixture A	Price
CS31041409	Emtricitabine System Suitability Mixture B	Price
CS31043207	Stavudine System Suitability Mixture	Price
CS3104409	Abacavir System Suitability Mixture	Price

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