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Quantification of trans-4-hydroxy-2-nonenal Enantiomers and Metabolites by LC-ESI-MS/MS

Quantification of trans-4-hydroxy-2-nonenal Enantiomers and Metabolites by LC-ESI-MS/MS

Ales Honzatko, Jiri Brichac, Matthew J Picklo

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 15;857(1):115-22.

PMID: 17662675

Abstract:

Lipid peroxidation is a causal factor in multiple diseases including Alzheimer's disease, atherosclerosis, and alcoholic liver disease. One of the most studied products of lipid peroxidation, trans-4-hydroxy-2-nonenal (HNE), has multiple cell signaling and cytotoxic effects. In this work, we developed an LC-MS/MS method for the quantitation of HNE enantiomers, the metabolite trans-4-hydroxy-2-nonenoic acid, and HNE-glutathione adducts in a single chromatographic run. In this method, (R)-HNE and (S)-HNE are derivatized by (S)-carbidopa to form diastereomers that are separated by a reversed-phase column. This method was successfully validated and tested using respiring rat brain mitochondria that enantioselectively metabolize HNE. Metabolic profiles of HNE biotransformation, including the enantiomeric disposition of HNE, will provide useful biomarker data regarding lipid peroxidation in disease states.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP28860959	S-(−)-Carbidopa		28860-95-9	Price

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