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Quantitative Analysis of Morphine in Dried Blood Spots by Using morphine-d3 Pre-Impregnated Dried Blood Spot Cards

Quantitative Analysis of Morphine in Dried Blood Spots by Using morphine-d3 Pre-Impregnated Dried Blood Spot Cards

John Mommers, Ynze Mengerink, Erik Ritzen, Jos Weusten, Jac van der Heijden, Sjoerd van der Wal

Anal Chim Acta. 2013 Apr 24;774:26-32.

PMID: 23567113

Abstract:

Two different internal standard dried blood spot (DBS) pre-impregnation procedures (prior to blood spotting) were investigated. In the first procedure DBS pre-impregnation is performed by immersing the DBS card fully into an internal standard solution. In the second procedure pre-impregnation is performed by pipetting a certain volume of an internal standard solution onto the DBS card. Morphine-d3 was used as the model compound for all experiments. The pre-impregnation procedure by immersing was further investigated with respect to homogeneity of impregnation, influence of different blood spotting techniques and the influence of spotting different blood volumes on the internal standard distribution, calibration and stability of pre-impregnated cards. Finally, the immersing procedure was used for the analysis of morphine in dried blood spots and the results were compared to the conventional procedure in which the internal standard morphine-d3 was added to the extraction solvent. The new pre-impregnated cards couple simplicity of operation and convenient use in the field to results equivalent to the conventional procedure.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP67293883	Morphine-D3 solution		67293-88-3	Price

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