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Quantitative Bioanalysis of Bavachalcone in Rat Plasma by LC-MS/MS and Its Application in a Pharmacokinetics Study

Quantitative Bioanalysis of Bavachalcone in Rat Plasma by LC-MS/MS and Its Application in a Pharmacokinetics Study

Dan Zhou, Lianhua An, Yan Xia, Yuanyi Wang, Xingliang Li

Biomed Chromatogr. 2017 Dec;31(12).

PMID: 28618051

Abstract:

This study aims to develop and validate a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for investigating the pharmacokinetic characteristics of bavachalcone. Liquid-liquid extraction was used to prepare plasma sample. Chromatographic separation of bavachalcone and IS was achieved using a Venusil ASB C18 (2.1 × 50 mm, 5 μm) column with a mobile phase of methanol (A)-water (B) (70:30, v/v). The detection and quantification of analytes was performed in selected-reaction monitoring mode using precursor → product ion combinations of m/z 323.1 → 203.2 for bavachalcone, and m/z 373.0 → 179.0 for IS. Linear calibration plots were achieved in the range of 1-1000 ng/mL for bavachalcone (r2 > 0.99) in rat plasma. The recovery of bavachalcone ranged from 84.1 to 87.0%. The method was precise, accurate and reliable. It was fully validated and successfully applied to pharmacokinetic study of bavachalcone.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP28448853	Bavachalcone		28448-85-3	Price

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