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Quantitative Bioimaging of Platinum Group Elements in Tumor Spheroids

Quantitative Bioimaging of Platinum Group Elements in Tumor Spheroids

Ann-Christin Niehoff, Jonas Grünebaum, Aline Moosmann, Dennis Mulac, Judith Söbbing, Rebecca Niehaus, Rebecca Buchholz, Sabrina Kröger, Arno Wiehe, Sylvia Wagner, Michael Sperling, Hagen von Briesen, Klaus Langer, etc.

Anal Chim Acta. 2016 Sep 28;938:106-13.

PMID: 27619092

Abstract:

Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14024614	Palladium(II) acetylacetonate		14024-61-4	Price

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