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Quantitative Detection of Fipronil and Fipronil-Sulfone in Sera of Black-Tailed Prairie Dogs and Rats After Oral Exposure to Fipronil by Camel Single-Domain Antibody-Based Immunoassays

Quantitative Detection of Fipronil and Fipronil-Sulfone in Sera of Black-Tailed Prairie Dogs and Rats After Oral Exposure to Fipronil by Camel Single-Domain Antibody-Based Immunoassays

Kai Wang, Natalia Vasylieva, Debin Wan, David A Eads, Jun Yang, Tyler Tretten, Bogdan Barnych, Ji Li, Qing X Li, Shirley J Gee, Bruce D Hammock, Ting Xu

Anal Chem. 2019 Jan 15;91(2):1532-1540.

PMID: 30521755

Abstract:

The insecticide fipronil can be metabolized to its sulfone in mammalian species. Two camel single-domain antibodies (VHHs) F1 and F6, selective to fipronil and fipronil-sulfone, respectively, were generated and used to develop enzyme linked immunosorbent assays (ELISAs) for the detection of the two compounds in the sera of black-tailed prairie dogs and rats. The limits of detection of fipronil and fipronil-sulfone in the rodent sera by the corresponding ELISAs were 10 and 30 ng mL-1, and the linear ranges were 30-1000 and 75-2200 ng mL-1. ELISAs showed a good recovery for fipronil and fipronil-sulfone cospiked in the control sera of the black-tailed prairie dogs (90-109%) and rats (93-106%). The VHH-based ELISAs detected fipronil and fipronil-sulfone in the sera of the rodents that received a repeated oral administration of fipronil. The average concentration of fipronil-sulfone was approximately 3.2-fold higher than fipronil in the prairie dog sera (1.15 vs 0.36 μg mL-1) and rat sera (1.77 vs 0.53 μg mL-1). ELISAs agreed well with a liquid chromatography-mass spectrometry method for the quantification of both fipronil and fipronil-sulfone in real serum samples. Fipronil-sulfone was identified as the predominant metabolite of fipronil in the black-tailed prairie dog and rat sera.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP120068362	Fipronil sulfone		120068-36-2	Price

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