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Radioimmunoassay of Free Genistein in Human Serum

Radioimmunoassay of Free Genistein in Human Serum

O Lapcík, R Hampl, M Hill, K Wähälä, N A Maharik, H Adlercreutz

J Steroid Biochem Mol Biol. 1998 Mar;64(5-6):261-8.

PMID: 9618027

Abstract:

Two radioimmunoassay (RIA) systems for genistein have been established, based on polyclonal antibodies against genistein-4'-O-(carboxymethyl)ether-bovine serum albumin and genistein-7-O-(carboxymethyl)ether-bovine serum albumin conjugates. The sensitivities of assays were 4.44 and 10.4 fmol (1.2 and 2.8 pg)/tube, respectively, the intraassay coefficients of variation ranged from 3.54 to 9.30%, the interassay C.V. varied from 6.72 to 19.7%, depending on the type of method and on genistein concentration. The cross-reactivities with other chemically related compounds (with exception of genistein derivatives at the position used for construction of the immunogen) were 5.5 and 6.1% for daidzein and 3.9 and 0.04% for formononetin in RIAs using reagents prepared through positions 4'- and 7- of genistein, respectively. The method was used for measurement of genistein levels in 26 omnivore subjects and in three volunteers after consumption of a meal prepared from 125 g of cooked whole soybeans. The values obtained in ether extracts from human sera were almost identical for both RIA systems, indicating that both RIAs measure the same entity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP446720-A	Genistein, Soybean - CAS 446-72-0		446-72-0	Price

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