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Radioiodinated 4-iodo-L-meta-tyrosine, a System L Selective Artificial Amino Acid: Molecular Design and Transport Characterization in Chinese Hamster Ovary Cells (CHO-K1 Cells)

Radioiodinated 4-iodo-L-meta-tyrosine, a System L Selective Artificial Amino Acid: Molecular Design and Transport Characterization in Chinese Hamster Ovary Cells (CHO-K1 Cells)

Naoto Shikano, Takashi Kotani, Syuichi Nakajima, Masato Ogura, Shinya Nakazawa, Jun-ichi Sagara, Masato Kobayashi, Takeshi Baba, Naoto Yamaguchi, Nobuo Kubota, Keiichi Kawai

Nucl Med Biol. 2010 Nov;37(8):903-10.

PMID: 21055620

Abstract:

Introduction:
High expression of the system L amino acid transporter has been observed in clinically important tissues including tumors and the blood-brain barrier. We examined amino acid transport system L selectivity of (14)C(U)-L-tyrosine ((14)C-Tyr), (125)I-4-iodo-L-meta-tyrosine (4-(125)I-mTyr), (125)I-6-iodo-L-meta-tyrosine (6-(125)I-mTyr), (125)I-3-iodo-α-methyl-L-tyrosine ((125)I-IMT) and (125)I-3-iodo-L-tyrosine (3-(125)I-Tyr) using Chinese hamster ovary cells (CHO-K1).
Methods:
Cells in the exponential growth phase were incubated with 18.5 kBq of labeled amino acid in 2 mL of phosphate-buffered saline-based uptake solution and an uptake solution with/without Na(+) at 37°C or 4°C. We examined the effects of the following compounds (1.0 mM) on transport: 2-(methylamino)isobutyric acid (a specific inhibitor of system A, in Na(+)-containing uptake solution); 2-amino-bicyclo[2,2,1]heptane-2-carboxylic acid (a specific inhibitor of system L, in Na(+)-free uptake solution); sodium azide and 2,4-dinitrophenol (NaN(3) and DNP, inhibitors of the generation of adenosine triphosphate); p-aminohippurate and tetraethylammonium (PAH and TEA, inhibitors of organic anion and cation transporters); and L- and D-isomers of natural amino acids.
Results:
(14)C-Tyr exhibited affinity for systems L, A and ASC. 4-(125)I-mTyr and 3-(125)I-Tyr exhibited high specificity for system L, whereas 6-(125)I-mTyr and (125)I-IMT exhibited affinity for both systems L and ASC. Uptake of 4-(125)I-mTyr was markedly reduced by incubation at 4 °C, and was not significantly inhibited by NaN(3), DNP, PAH or TEA. The inhibition profiles of the L- and D-isomers of natural amino acids indicated that system L mediates the transport of 4-(125)I-mTyr.
Conclusions:
4-(125)I-mTyr exhibited the greatest system L specificity (93.46 ± 0.13%) of all of the tested amino acids.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP672877-B	α-Methyl-L-tyrosine		672-87-7	Price
AP70780	3-Iodo-L-tyrosine		70-78-0	Price

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