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Rapid and Sensitive Plate Method for Detection of Aspergillus Fumigatus

Rapid and Sensitive Plate Method for Detection of Aspergillus Fumigatus

T G Bauters, H J Nelis

J Clin Microbiol. 2000 Oct;38(10):3796-9.

PMID: 11015405

Abstract:

The routine identification of Aspergillus fumigatus in clinical samples involves, apart from direct examination, the isolation of the organism on a plate followed by its microscopic characterization. This approach lacks sensitivity, specificity, and speed. A new procedure has been developed combining microcolony formation on a nylon membrane filter at 45 degrees C with the detection of a specific 4-methylumbelliferyl-alpha-L-arabinopyranoside cleaving enzyme activity in digitonin permeabilized cells. The test takes approximately 14 h and has an efficiency of 98.2% and false-positive and -negative rates of 0 and 3.1%, respectively. When applied to 188 clinical samples taken from patients with proven or nonproven presence of Aspergillus species, a good agreement with the conventional plate-microscopy method was obtained.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP69414262	4-Methylumbelliferyl α-L-arabinopyranoside		69414-26-2	Price

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