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Rapid Centrifugation in the Routine Hemostasis Laboratory

Rapid Centrifugation in the Routine Hemostasis Laboratory

Nathan Wolfensberger, Georgios Georgiou, Evelyne Giabbani, Marianne Reusser, Linet M Njue, Martin Fiedler, Alexander B Leichtle, Michael Nagler

Thromb Haemost. 2019 Dec;119(12):2025-2033.

PMID: 31600807

Abstract:

Background:
The use of short and uniform centrifugation schemes contributes significantly to the successful automation of laboratory procedures. It is however unclear if this is applicable to the hemostasis laboratory.
Objectives:
This article assesses the accuracy of measurements obtained with a rapid, high-speed centrifugation scheme in a large set of hemostasis tests, covering the full spectrum of values obtained in clinical practice, and using meaningful statistical measures.
Methods:
Two citrated plasma samples were obtained from consecutive patients of a tertiary hospital with suspected abnormal hemostasis tests and processed with two centrifugation schemes in parallel: 1,500 × g for 10 minutes and 3,137 × g for 7 minutes. The following tests were conducted: prothrombin time (n = 125), international normalized ratio (n = 146), activated partial thromboplastin time (n = 119), thrombin time (n = 105), fibrinogen (n = 125), factor (F)II (n = 69), FV (n = 64), FVII (n = 64), FX (n = 67), FVIII (n = 55), FIX (n = 37), FXI (n = 35), and FXIII (n = 20), D-dimer (n = 34), antithrombin (n = 31), anti-Xa activity (n = 30), von Willebrand antigen (n = 25), and von Willebrand activity (VWF:GPIbM; n = 27).
Results:
A wide range of results were obtained in all tests. Spearman's rank correlation coefficient was at least 0.95 for all tests except FV, FIX, and FXI. The coverage probability π at a given deviation index κ of 15% was above 0.9 for all tests except FV, FVII, FX, FVIII, FIX, FXI, and VWF:GPIbM, suggesting a lack of agreement.
Conclusion:
Our results suggest that high-speed centrifugation is applicable to the majority of routine hemostasis parameters. The coverage probability was more sensitive than Spearman's rank correlation to detect disagreement among centrifugation schemes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP205503613	N,N′,N′′-Tri-tert-butylsilanetriamine		205503-61-3	Price

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