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Rapid Imaging, Using a Cooled Charge-Coupled-Device, of Fluorescent Two-Dimensional Polyacrylamide Gels Produced by Labelling Proteins in the First-Dimensional Isoelectric Focusing Gel With the Fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone

Rapid Imaging, Using a Cooled Charge-Coupled-Device, of Fluorescent Two-Dimensional Polyacrylamide Gels Produced by Labelling Proteins in the First-Dimensional Isoelectric Focusing Gel With the Fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone

P Jackson, V E Urwin, C D Mackay

Electrophoresis. 1988 Jul;9(7):330-9.

PMID: 2466662

Abstract:

A new method for visualising proteins in two-dimensional polyacrylamide gels was developed. Proteins were labelled with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone (MDPF) while present in the first-dimensional gel after isoelectric focusing and subsequently electrophoresed into the second-dimensional gel. High resolution spot patterns were produced and compared with other methods of visualisation. A new rapid imaging system based on a cooled charge-coupled-device was used to view the two-dimensional fluorescent protein spot patterns. The method allows the immediate and rapid imaging of two-dimensional gels at the end of electrophoresis with no further processing.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP50632570	2-Methoxy-2,4-diphenyl-3(2H)-furanone		50632-57-0	Price

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