Home
Resources
Publications
Rapid Method for Protein Quantitation by Bradford Assay After Elimination of the Interference of Polysorbate 80

Rapid Method for Protein Quantitation by Bradford Assay After Elimination of the Interference of Polysorbate 80

Yongfeng Cheng, Haiming Wei, Rui Sun, Zhigang Tian, Xiaodong Zheng

Anal Biochem. 2016 Feb 1;494:37-9.

PMID: 26545323

Abstract:

Bradford assay is one of the most common methods for measuring protein concentrations. However, some pharmaceutical excipients, such as detergents, interfere with Bradford assay even at low concentrations. Protein precipitation can be used to overcome sample incompatibility with protein quantitation. But the rate of protein recovery caused by acetone precipitation is only about 70%. In this study, we found that sucrose not only could increase the rate of protein recovery after 1 h acetone precipitation, but also did not interfere with Bradford assay. So we developed a method for rapid protein quantitation in protein drugs even if they contained interfering substances.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9005656-A	Polysorbate 80		9005-65-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: