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Rat interleukin-18 Binding Protein: Cloning, Expression, and Characterization

Sin-Hyeog Im, Soo-Hyun Kim, Tania Azam, Natarajan Venkatesh, Charles A Dinarello, Sara Fuchs, Miriam C Souroujon

J Interferon Cytokine Res. 2002 Mar;22(3):321-8.

PMID: 12034039

Abstract:

Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-gamma (IFN-gamma) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42413369 Interleukin-18 from rat Interleukin-18 from rat Price
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