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Rate-limiting Steps for the Esterase and Dehydrogenase Reaction Catalyzed by Horse Liver Aldehyde Dehydrogenase

Rate-limiting Steps for the Esterase and Dehydrogenase Reaction Catalyzed by Horse Liver Aldehyde Dehydrogenase

H Weiner, J H Hu, C G Sanny

J Biol Chem. 1976 Jul 10;251(13):3853-5.

PMID: 945270

Abstract:

Horse liver aldehyde dehydrogenase, like other aldehyde dehydrogenases, is capable of hydrolyzing esters such as nitrophenyl acetate. Pre-steady state and burst kinetics were performed using substrate levels of enzyme to determine whether the rate-limiting step occurred prior to or after the formation of an acyl enzyme intermediate. A burst was found by both techniques for the dehydrogenase reaction; but no burst was found for the esterase reaction. These data show that the rate-limiting step for the two reactions catalyzed by the enzyme differs. For dehydrogenase it occurs after the formation of the acyl intermediate, but for esterase it occurs prior to its formation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9016186-D	Esterase from horse liver		9016-18-6	Price

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