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Reciprocal Modulation of anti-IgE Induced Histamine Release From Human Mast Cells by A₁ and A(2B) Adenosine Receptors

Reciprocal Modulation of anti-IgE Induced Histamine Release From Human Mast Cells by A₁ and A(2B) Adenosine Receptors

K H Yip, H Y A Lau, H Wise

Br J Pharmacol. 2011 Sep;164(2b):807-19.

PMID: 21506953

Abstract:

Background and purpose:
Adenosine is believed to participate in the pathological development of asthma through a mast cell-dependent mechanism. Our study aimed to pharmacologically characterize the functions of adenosine receptor (AR) subtypes (A₁, A(2A) , A(2B) and A₃) in primary human cultured mast cells (HCMC).
Experimental approach:
HCMC were derived from progenitor stem cells in buffy coat and the effects of adenosine receptor ligands on basal and IgE-dependent histamine release were evaluated.
Key results:
Adenosine and analogues alone did not induce HCMC degranulation. When HCMC were activated by anti-IgE after 10 min pre-incubation with adenosine, a biphasic effect on histamine release was observed with enhancement of HCMC activation at low concentrations of adenosine (10⁻⁹-10⁻⁷ mol·L⁻¹) and inhibition at higher concentrations (10⁻⁶-10⁻⁴ mol·L⁻¹). The potentiating action was mimicked by A₁ AR agonists CCPA and 2'MeCCPA, and inhibited by the A₁ AR antagonist PSB36. In contrast, the inhibitory action of adenosine was mimicked by the non-specific A₂ AR agonist CV1808 and attenuated by A(2B) AR antagonists PSB1115 and MRS1760. The non-selective AR antagonist CGS15943 attenuated both the potentiating and inhibitory actions.
Conclusions and implications:
We have defined for the first time the contribution of A₁ and A(2B) ARs, respectively, to the potentiating and inhibitory action of adenosine on human mast cell activation. With reference to the current trend of developing novel anti-asthmatic agents from AR ligands, our results suggest that inhibition of human mast cell activation would be a mechanism for A₁ AR antagonists, but not A(2B) AR antagonists.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP524944727	PSB36		524944-72-7	Price

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