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Recombinant Escherichia Coli Engineered for Production of L-lactic Acid From Hexose and Pentose Sugars

Recombinant Escherichia Coli Engineered for Production of L-lactic Acid From Hexose and Pentose Sugars

B S Dien, N N Nichols, R J Bothast

J Ind Microbiol Biotechnol. 2001 Oct;27(4):259-64.

PMID: 11687940

Abstract:

Recombinant Escherichia coli have been constructed for the conversion of glucose as well as pentose sugars into L-lactic acid. The strains carry the lactate dehydrogenase gene from Streptococcus bovis on a low copy number plasmid for production of L-lactate. Three E. coli strains were transformed with the plasmid for producing L-lactic acid. Strains FBR9 and FBR11 were serially transferred 10 times in anaerobic cultures in sugar-limited medium containing glucose or xylose without selective antibiotic. An average of 96% of both FBR9 and FBR11 cells maintained pVALDH1 in anaerobic cultures. The fermentation performances of FBR9, FBR10, and FBR11 were compared in pH-controlled batch fermentations with medium containing 10% w/v glucose. Fermentation results were superior for FBR11, an E. coli B strain, compared to those observed for FBR9 or FBR10. FBR11 exhausted the glucose within 30 h, and the maximum lactic acid concentration (7.32% w/v) was 93% of the theoretical maximum. The other side-products detected were cell mass and succinic acid (0.5 g/l).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001609-A	Lactic Dehydrogenase, recombinant from E. coli		9001-60-9	Price

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