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Reduced Graphene Oxide Field-Effect Transistor for Label-Free Femtomolar Protein Detection

Reduced Graphene Oxide Field-Effect Transistor for Label-Free Femtomolar Protein Detection

Duck-Jin Kim, Il Yung Sohn, Jin-Heak Jung, Ok Ja Yoon, N-E Lee, Joon-Shik Park

Biosens Bioelectron. 2013 Mar 15;41:621-6.

PMID: 23107386

Abstract:

We report reduced graphene oxide field effect transistor (R-GO FET) biosensor for label-free ultrasensitive detection of a prostate cancer biomarker, prostate specific antigen/α1-antichymotrypsin (PSA-ACT) complex. The R-GO channel in the device was formed by reduction of graphene oxide nanosheets networked by a self-assembly process. Immunoreaction of PSA-ACT complexes with PSA monoclonal antibodies on the R-GO channel surface caused a linear response in the shift of the gate voltage, V(g,min), where the minimum conductivity occurs. The R-GO FET can detect protein-protein interactions down to femtomolar level with a dynamic range over 6-orders of magnitude in the V(g,min) shift as a sensitivity parameter. High association constants of 3.2 nM(-1) and 4.2 nM(-1) were obtained for the pH 6.2 and pH 7.4 analyte solutions, respectively. The R-GO FET biosensor showed a high specificity to other cancer biomarker in the phosphate buffered saline solutions as well as in the human serum.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414845	Prostate Specific Antigen-α1-Antichymotrypsin Complex human			Price

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