Home
Resources
Publications
Reduction of Aspergillus Niger Virulence in Apple Fruits by Deletion of the Catalase Gene cpeB

Reduction of Aspergillus Niger Virulence in Apple Fruits by Deletion of the Catalase Gene cpeB

Meng-Ke Zhang, Jun Tang, Zhong-Qin Huang, Kang-Di Hu, Yan-Hong Li, Zhuo Han, Xiao-Yan Chen, Lan-Ying Hu, Gai-Fang Yao, Hua Zhang

J Agric Food Chem. 2018 May 30;66(21):5401-5409.

PMID: 29745230

Abstract:

Aspergillus niger, a common saprophytic fungus, causes rot in many fruits. We studied the role of a putative catalase-peroxidase-encoding gene, cpeB, in oxidative stress and virulence in fruit. The cpeB gene was deleted in A. niger by homologous recombination, and the Δ cpeB mutant showed decreased CAT activity compared with that of the wild type. The cpeB gene deletion caused increased sensitivity to H2O2 stress, and spore germination was significantly reduced; in addition, the reactive-oxygen-species (ROS) metabolites superoxide anions (·O2-), hydrogen peroxide (H2O2), and malondialdehyde (MDA) accumulated in the Δ cpeB mutant during H2O2 stress. Furthermore, ROS metabolism in A. niger infected apples was determined, and our results showed that the Δ cpeB mutant induced an attenuated response in apple fruit during the fruit-pathogen interaction; the cpeB gene deletion significantly reduced the development of lesions, suggesting that the cpeB gene in A. niger is essential for full virulence in apples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001052-A	Catalase from Aspergillus niger		9001-05-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: