Home
Resources
Publications
Reduction of Dihydrokaempferol by Vitis Vinfera Dihydroflavonol 4-Reductase to Produce Orange Pelargonidin-Type Anthocyanins

Reduction of Dihydrokaempferol by Vitis Vinfera Dihydroflavonol 4-Reductase to Produce Orange Pelargonidin-Type Anthocyanins

Sha Xie, Ting Zhao, Zhenwen Zhang, Jiangfei Meng

J Agric Food Chem. 2018 Apr 4;66(13):3524-3532.

PMID: 29554804

Abstract:

Vitis vinifera has been thought to be unable to produce pelargonidin-type anthocyanins because its dihydroflavonol 4-reductase (DFR) does not efficiently reduce dihydrokaempferol. However, in this study, pelargonidin 3- O-glucoside was detected in the skins of V. vinifera 'Pinot Noir', 'Cabernet Sauvignon', and 'Yan73', as well as in the flesh of 'Yan73' by HPLC-ESI-MS/MS. Additionally, pelargonidin 3- O-(6-acetyl)-glucoside was detected in 'Yan73' skin and flesh for the first time. To further confirm the presence of pelargonidin-type anthocyanins in these grape cultivars, their DFRs were cloned, expressed in Escherichia coli, and purified. An enzyme-activity analysis revealed that V. vinifera DFR can reduce dihydrokaempferol to produce leucopelargonidin, although it prefers dihydroquercetin and dihydromyricetin as substrates. Thus, the existence of a pelargonidin-based anthocyanin-biosynthetic pathway was confirmed in V. vinifera via mass-spectrometric and enzymatic methods and redirected anthocyanin biosynthesis in V. vinifera L. cultivars.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP480206	Dihydrokaempferol		480-20-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: