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Reductively Degradable Poly(2-hydroxyethyl Methacrylate) Hydrogels With Oriented Porosity for Tissue Engineering Applications

Reductively Degradable Poly(2-hydroxyethyl Methacrylate) Hydrogels With Oriented Porosity for Tissue Engineering Applications

Hana Macková, Zdeněk Plichta, Helena Hlídková, Ondřej Sedláček, Rafal Konefal, Zhansaya Sadakbayeva, Miroslava Dušková-Smrčková, Daniel Horák, Šárka Kubinová

ACS Appl Mater Interfaces. 2017 Mar 29;9(12):10544-10553.

PMID: 28287694

Abstract:

Degradable poly(2-hydroxyethyl methacrylate) hydrogels were prepared from a linear copolymer (Mw = 49 kDa) of 2-hydroxyethyl methacrylate (HEMA), 2-(acethylthio)ethyl methacrylate (ATEMA), and zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC). The deprotection of ATEMA thiol groups by triethylamine followed by their gentle oxidation with 2,2'-dithiodipyridine resulted in the formation of reductively degradable polymers with disulfide bridges. Finally, a hydrogel 3D structure with an oriented porosity was obtained by gelation of the polymer in the presence of needle-like sodium acetate crystals. The pore diameter and porosity of resulting poly(2-hydroxyethyl methacrylate-co-2-(acethylthio)ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] hydrogels varied between 59 and 65 μm and between 70 and 79.6 vol % according to Hg porosimetry, and complete degradation of these materials was reached in 86 days in 0.33 mmol solution of l-cysteine/L in phosphate buffer. The cross-linked P(HEMA-ATEMA-MPC) hydrogels were evaluated as a possible support for human mesenchymal stem cells (MSCs). No cytotoxicity was found for the un-cross-linked thiol-containing and protected P(HEMA-ATEMA-MPC) chains up to a concentration of 5 and 1 wt % in α-minimum essential medium, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25249165	Poly(2-hydroxyethyl methacrylate)		25249-16-5	Price
LS761043	Poly[(propylmethacryl-heptaisobutyl-PSS)-co-hydroxyethyl methacrylate]			Price

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