Home
Resources
Publications
Regulation of Human Growth and Differentiation Factor 3 Gene Expression by NANOG in Human Embryonic Carcinoma NCCIT Cells

Regulation of Human Growth and Differentiation Factor 3 Gene Expression by NANOG in Human Embryonic Carcinoma NCCIT Cells

Sung-Won Park, Hye-Young Lim, Hyun-Jin Do, BoReum Sung, Sun-Hyung Huh, Sang-Jun Uhm, Hyuk Song, Hak-Jae Chung, Jin-Hoi Kim, Nam-Hyung Kim, Jae-Hwan Kim

FEBS Lett. 2012 Sep 21;586(19):3529-35.

PMID: 22963770

Abstract:

We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was activated by NANOG, and the upstream region (-183 to -1) was sufficient to induce minimal transcriptional activity. Moreover, NANOG binds to the GDF3 minimal promoter in vivo and the transcriptional activity is mediated by NANOG transactivation domain. This study provides the first evidence that NANOG is a transcriptional activator of the expression of the oncogenic growth factor GDF3 in embryonic carcinoma cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413736	GDF-3 human			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: