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Regulation of IGF-I and -II by Insulin in Primary Cultures of Fetal Rat Hepatocytes

Regulation of IGF-I and -II by Insulin in Primary Cultures of Fetal Rat Hepatocytes

L Goya, A de la Puente, S Ramos, M A Martín, F Escrivá, C Alvarez, A M Pascual-Leone

Endocrinology. 2001 Dec;142(12):5089-96.

PMID: 11713201

Abstract:

During perinatal development, insulin and nutrients, rather than GH, regulate the IGF system. A selective primary culture of fetal rat hepatocytes has been established in our laboratory to elucidate the molecular mechanism of action of the above regulatory factors on IGF-I and -II gene expression during the late fetal period of the rat. In this model we have previously reported a regulatory role for glucose on IGF-I and -II synthesis and secretion. In the same experimental model, we now report that doses of insulin (0.1-5 microM) within the physiological range in rat fetuses during the last stages of gestation evoke an increase of IGF-I and -II mRNA abundance. Insulin regulated in a parallel manner IGF peptide secretion, and an excellent correlation was observed between IGF-I and -II mRNA and IGF-I and -II peptide levels in the conditioned media in response to the hormone. Finally, the insulin-induced rise in IGF-I and -II mRNA was not mediated by stimulation of gene transcription but by increased transcript stability. The results support the hypothesis that insulin plays a major role in IGF regulation at immature stages of development.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248159	IGF-I from rat			Price

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