Home
Resources
Publications
Removal of Trans-2-nonenal Using Hen Egg White Lysosomal-Related Enzymes

Removal of Trans-2-nonenal Using Hen Egg White Lysosomal-Related Enzymes

Seyoung Lee, Ji Hun Kim, Ngoc-Tu Nguyen, Ra-Mi Park, Su-Min Lee, Seung Hyuck Bang, Gyeongchan Jeon, Jaewoong Lee, Sunchang Kim, Byung-Kwan Cho, Yang-Hoon Kim, Jiho Min

Mol Biotechnol. 2020 Jun 24.

PMID: 32583365

Abstract:

2-Nonenal is a long-chain aliphatic aldehyde containing nine carbons and an unsaturated bond. 2-Nonenal is the primary cause of odor associated with aging, with an unpleasant greasy and grassy odor. Lysosome, mitochondria, and peroxisome are significant organelles in eukaryotic cells that contain various hydrolases that degrade biomolecules. Proteins in mitochondria and peroxisome also contain aldehyde dehydrogenase. We performed trans-2-nonenal treatment using lysosomal-related enzymes extracted from hen egg white (HEW). As trans-2-nonenal is more structurally stable than cis-2-nonenal, it was selected as the target aldehyde. HEW contains various biologically active proteins and materials such as albumin, ovotransferrin, lysosome, peroxisome, and mitochondria. Here, complementary experiments were conducted to evaluate the role of lysosomal-related enzymes in the treatment of trans-2-nonenal. The activity of lysosomal-related enzymes was confirmed via antimicrobial test against E. coli. HPLC analysis was used to determine the reduction of trans-2-nonenal. The trans-2-nonenal treatment depended on the reaction time and enzyme concentration. Materials considered as an intermediate from trans-2-nonenal treatment were detected by GC/MS spectrometer. Under acidic conditions (pH 6), lysosomal-related enzymes were the most efficient in the treatment of trans-2-nonenal. Furthermore, based on differential pH testing, we found the conditions under which all the 50 ppm trans-2-nonenal was removed. Therefore, our results suggest that the lysosomal-related enzymes reduced trans-2-nonenal, suggesting clinical application as anti-aging deodorants.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18829566	trans-2-Nonenal		18829-56-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: