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[Research on Period of Validity of Emodin Standard Solution and Chrysophanol Standard Solution]

[Research on Period of Validity of Emodin Standard Solution and Chrysophanol Standard Solution]

Hui-Jun Gou, Ying Yang, Jie Ren, Jun Lu, Yuan Zheng, Ting Li

Zhongguo Zhong Yao Za Zhi. 2018 Dec;43(24):4892-4899.

PMID: 30717536

Abstract:

In order to study the storage and period of validity of emodin standard solution and chrysophanol standard solution in this study, the content of emodin and chrysophanol was determined by HPLC through classical constant temperature test, and the change rule of the content of the standard solution was studied, which could be applied to standardize the management of the standard substance of traditional Chinese medicine. The results showed that the content of emodin and chrysophanol standard solution matched with the first order reaction rule. Under the storage condition of 10 °C, the change rate constant of emodin and chrysophanol were Ke=4.661 7×10⁻⁷ and Kc=4.438 9×10⁻⁷, respectivedy; and the period of validity of emodin standard solution and chrysophanol standard solution were 1 806 d and 1 896 d respectively. The determination and standardization of the period of validity of the standard solution will not only help to reduce the loss of the standard substance and save the cost of drug testing, but also help to standardize the use of the standard substance, which will contrite to obtain more accurate and satisfactory experimental results, and provide a basis for the setting of the storage period and standardized management of the reference solution of Chinese medicine.

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