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Resolution of Epoxide Enantiomers of Polycyclic Aromatic Hydrocarbons by Chiral Stationary-Phase High-Performance Liquid Chromatography

H B Weems, M Mushtaq, S K Yang

Anal Biochem. 1985 Aug 1;148(2):328-38.

PMID: 4061813

Abstract:

Enantiomers of nine K-region and one non-K-region epoxides of polycyclic aromatic hydrocarbons have been resolved by high-performance liquid chromatography with chiral stationary phases either ionically or covalently bonded to gamma-aminopropylsilanized silica. Resolution of enantiomers was confirmed by ultraviolet-visible absorption, circular dichroism, and mass spectral analyses. This method has been applied to the determination of optical purity and absolute configuration of the K-region epoxides formed in the metabolism of 1-methylbenz[a]anthracene, 7-methylbenz[a]anthracene, and 12-methylbenz[a]anthracene by rat liver microsomes.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP2498773 1-Methylbenz[a]anthracene 1-Methylbenz[a]anthracene 2498-77-3 Price
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