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Resolution of Salbutamol Enantiomers in Human Urine by Reversed-Phase High Performance Liquid Chromatography After Derivatization With (S)-(-)-alpha-methylbenzyl Isocyanate

K H Kim, T K Kim, Y H Kwon, Y T Sohn

Arch Pharm Res. 1997 Oct;20(5):486-90.

PMID: 18982495

Abstract:

A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)-alpha-methylbenzyl isocyanate, the diastereomeric derivatives were resolved (R(s)=1.59) on 5 mum octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP14649037 (S)-(−)-α-Methylbenzyl isocyanate (S)-(−)-α-Methylbenzyl isocyanate 14649-03-7 Price
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