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Retinoids and Malignant Melanoma: A Pathway of Proliferation Inhibition on SK MEL28 Cell Line

Retinoids and Malignant Melanoma: A Pathway of Proliferation Inhibition on SK MEL28 Cell Line

Laura Emionite, Fabia Galmozzi, Patrizia Raffo, Laura Vergani, Salvatore Toma

Anticancer Res. Jan-Feb 2003;23(1A):13-9.

PMID: 12680189

Abstract:

Background:
It has been observed, in preclinical and clinical studies, that retinoids may interfere with the carcinogenic process in different ways such as the control of proliferation, differentiation and apoptosis.
Materials and methods:
We evaluated the in vitro efficacy of some synthetic retinoids (RAR-alpha, beta and gamma; RXR-alpha agonists and RAR-alpha antagonist) and compared these to the panagonist all-trans retinoic acid in inhibiting growth of the human melanoma cell line, SK MEL 28. We estimated, in parallel, apoptosis as a function of the treatment and, by RT-PCR, we analysed the effects of retinoids on the transcriptional profile of relevant genes, such as retinoid receptors and regulatory proteins.
Results:
We observed a marked antiproliferative rate with the RAR-gamma selective agonist RO 44-4753 and the RAR-alpha selective antagonist RO 41-5253; on the contrary, the other synthetic retinoids exhibited a rather low efficacy. All the tested retinoids appeared to activate the RAR-beta gene and major expression was evidenced following RO 44-4753 and RO 41-5253 treatment.
Conclusion:
Among the tested retinoids, RO 41-5253 exhibited marked effects on proliferation and RARs mRNA expression and its action appeared mainly related to a cell-cycle arrest rather than an apoptotic mechanism.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP144092319	Ro 41-5253		144092-31-9	Price

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