Home
Resources
Publications
Retroviral Endostatin Gene Transfer Inhibits Growth of Human Lung Cancer in a Murine Orthotopic Xenotransplant Model

Retroviral Endostatin Gene Transfer Inhibits Growth of Human Lung Cancer in a Murine Orthotopic Xenotransplant Model

Roland Kurdow, Arnd S Boehle, Maren Ruhnke, Renata Mendoza, Lars Boenicke, Bence Sipos, Bodo Schniewind, Peter Dohrmann, Holger Kalthoff

Langenbecks Arch Surg. 2003 Dec;388(6):401-5.

PMID: 12898260

Abstract:

Background and aims:
The administration of endostatin, a potent anti-angiogenic agent, will be required for extended periods of time as a cancer treatment. The aim of the present study was to induce endogenous endostatin secretion in a continuous fashion, based on retroviral gene transfer. The tumor response was evaluated in an orthotopic murine tumor model of human lung cancer.
Materials and methods:
Human non-small-cell lung cancer cells (KNS 62) were retrovirally transduced with the human endostatin gene. An orthotopic murine xenotransplant model was used to investigate tumor growth, metastases and survival. After 4 weeks of subcutaneous growth, endostatin expression was measured by immunoblot analysis in tumor lysates.
Results:
The growth of the subcutaneous tumors was significantly delayed, and orthotopic tumor growth and pleural metastases were significantly reduced in endostatin-transduced KNS 62 tumors. Prolongation of survival subsequent to orthotopic tumor induction was demonstrated. Strong endostatin expression was found in subcutaneous tumors after 4 weeks.
Conclusion:
Retroviral transduction of the human endostatin gene is capable of achieving long-term endostatin expression. Endostatin transduction provides significant anti-tumor effects with regard to local tumor growth, metastases and survival.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413060	Endostatin murine			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: